The present study was carried out to study the effects of Rhododendron arboreum extract on pharmacokinetic of isradipine in rats. Albino wistar rats (200-250 g) were used for pharmacokinetic study. Rats were divided into three groups. Group I were treated with only isradipine (1 mg/kg, p.o.). Group 2 and 3 were treated with Rhododendron arboreum extract (RAE) (100 and 200 mg/kg, p.o., respectively) 15 minute before administration of isradipine (1 mg/kg, p.o). Blood samples were collected at different time intervals from each animal. Plasma was separated from blood. Plasma concentrations of isradipine were measured by HPLC method. Pharmacokinetic parameters such as area under curve (AUC), peak plasma concentration (Cmax), time to occur peak plasma concentration (Tmax) and half-life (T1/2) were calculated. Animals were treated with RAE followed by isradipine significantly (P<0.05) increased AUC as compared to animals treated with only isradipine. The Cmax of Isradipine without and with RAE (100 and 200 mg/kg) was found to be 2044±9.945 ng/l, 3060.5±12.29 ng/l and 387.06±1.37 ng/l respectively. Tmax of isradipine without and with RAE (100 and 200 mg/kg) was found to be 1 and 2 hrs respectively. The half-life of isradipine without and with RAE (100 and 200 mg/kg) was found to be 6.18±0.091, 7.46±0.097 and 8.41±0.147 respectively. Cmax, Tmax and AUC after oral administration of isradipine were significantly increased by simultaneous oral treatment with RAE respectively. The results of present investigation showed that RAE significantly increased bioavailability of isradipine on dose depended manner.
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